Figure 2. Sequence map of the AHPND-associated plasmid pVH_vo in V. owensii and sequence identity plot of pVH plasmids.

All predicted open reading frames (ORFs) are shown as arrows in different colors. Direction of arrowhead represents the transcriptional orientation. The pndA and mobilization genes are labeled with red asterisks. (A) Sequence identity plot (>99%) of pVH_vo (gray), pVPA3-1 (blue) and pVA1 (blue, inner circle). (B) Sequence identity plot (>99%) of pVH_vo (gray), pVH_vh1 (green), pVH_vp1-8 (blue, outer to inner). The sequence of pVH_vo was used as reference.