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. 2017 Feb 7;7:42177. doi: 10.1038/srep42177

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Copyright © 2017, The Author(s)

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(A) Phylogeny of PirB in Vibrio and its homologs. The Maximum Likelihood tree was rooted using the juvenile hormone esterase-related protein of Leptinotarsa decemlineata as outgroup. Vibrio and Shewanella are indicated in bold. Multiple sequence alignments were performed with MUSCLE. The robustness of the tree was evaluated using bootstrap analysis (1,000). Percentage values are indicated at the nodes. Bar = 20 substitutions per 100 amino acids. (B) Phylogeny of the IS903 transposase encoded by pVH plasmids and its homologues. The Maximum Likelihood tree was rooted using E. coli IS903 transposase as outgroup. Vibrio is indicated in bold, and Shewanella in blue. Multiple sequence alignments were performed by using MUSCLE. The robustness of the tree was evaluated using bootstrap analysis (1,000). Percentage values are indicated at the nodes. Bar = 10 substitutions per 100 amino acids.