Figure 1. Cytokine responses were measured by assaying peripheral blood mononuclear cells for 48 h with heat-killed Mycobacterium avium complex (MAC) bacilli (multiplicity of infection [MOI]: 20 and 100), MAC sensitin (10 μg/ml), and phytohemaglutinin-L (PHA) (2.5 ng/ml) in patients with MAC-lung disease (LD) and controls.

The samples from all of the enrolled subjects underwent MAC MOI100 and PHA stimulation, and the number of samples assayed for other antigens are described in the Supplement file. Values for (A) tumor necrosis factor-alpha (TNF-α) and (B) interferon-gamma (IFN-γ) are presented as dot plots with crossed lines of mean values, and were analyzed using the Student’s t test. *0.01 ≤ p < 0.05; **0.001 < p < 0.01; ***p < 0.001.