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. 2017 Feb 7;7:42056. doi: 10.1038/srep42056

Figure 3. In silico promoter analysis of the 434 bp TERRA sequence from the subtelomere of human chromosome 15q.

Figure 3

(A) The PromoterInspector (Genomatix) predicted core promoter is displayed in blue and underlined with a blue bar. Matches to the MatInspector (Genomatix) predicted Retinoblastoma control elements (RCEs) are marked in green. The strand orientations are indicated by the underlining green arrows. The 5′ Xho II and the 3′ HindIII restriction sites recognition sequences are shown in red. (B) Luciferase expression of all the constructs cloned into pGL3 used in this study. The pGL3 vector was used as a positive control (black bar). The promotorless vector (pGL3-SV40) was unable to drive luciferase expression and used as negative control. The full length TERRA promoter vector (vTERRA) drove luciferase expression (grey bar). The expression under this promoter was significantly stronger than the SV40 promoter present in the pGL3 control vector. The truncated version of the TERRA promoter (vTERRA*) was not able to drive luciferase expression. RLU stands for relative luciferase units (relative to β-Galactosidase). Error bars show the SD of 3 technical replicates.