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. 2017 Feb 7;7:41576. doi: 10.1038/srep41576

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Copyright © 2017, The Author(s)

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(A,B) The average number of LS174T-EPHA3 (A) and DLD1-EPHA3 (B) cells and the corresponding empty vector control (EV) at the indicated times after seeding is shown (mean ± SEM of three independent experiments). (C,D) Number of colonies of LS174T-EPHA3 (C) and DLD1-EPHA3 (D) cells and the corresponding empty vector control (EV) grown on a solid plastic substrate (left), or on soft agar (right) with or without doxycycline treatment (1 μg/ml). The mean ( ±SEM) of three independent experiments run in triplicate is shown. (E,F) Growth of LS174T-EPHA3 (E) and DLD1-EPHA3 (F) cells when injected subcutaneously in immunodeficient NOD/SCID mice. Doxycycline was administered to the indicated groups of animals in the drinking water. The average tumor size ( ±SEM) is shown. N = 6 animals per group.