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. 2017 Feb 6;14:10. doi: 10.1186/s12977-017-0337-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Modification of the C element abolishes gag and pol expression but C element is not essential for RNA export. a Subcellular distribution of foamy viral transcripts was analysed by northern blotting using a tas-specific probe. bet is expressed from a spliced transcript encompassing parts of the tas and bel2 gene [15, 20]. b Influences of the pol elements located in the SpeI- and SexAI-fragments on Gag expression were analysed by Western blotting with monoclonal anti-Gag antibodies. The position of the C element is indicated (blue box). Detection of GAPDH served as loading control. Samples were analysed in independent duplicates