Fig. 4.

Mechanical allodynia and thermal hyperalgesia in CD8⁺-depleted arthritic mice. Prior to arthritis induction, CD8⁺ cells were depleted by repeated i.p. injections of anti-CD8 antibodies (clone 2.43) until the end of the observation period. Control mice received the isotype-matched control antibody. a Success of CD8⁺ depletion was confirmed in the peripheral blood by flow cytometry. Paw withdrawal thresholds and paw withdrawal latencies of CD8⁺-depleted or isotype-treated arthritic mice were determined during the onset of chronic ACIA at the time points indicated in the ipsi- and contralateral paws using von Frey filaments (b) and Hargreaves test (c), respectively. Data are represented as means ± SEM. Statistical analysis was performed using the two-way RM ANOVA and Bonferroni’s multiple comparison test followed by a Bonferroni correction comparing ipsi- vs. contralateral sides of animals receiving anti-CD8 (***P < 0.001) or a control antibody (^### P < 0.001); no significant differences were observed between ipsilateral thresholds of anti-CD8 and isotype control antibody-treated animals or between the contralateral paws of the two treatment groups, N = 8