Fig. 11.

Effect of TLR agonists in combination with pro-inflammatory stimuli in HIV reactivation in hµglia/HIV (HC01) cells. Flow cytometry analysis of the effect of poly (I:C) or imiquimod treatment in combination with TNF-α or IL-1β on HIV emergence from latency. a hµglia/HIV (HC01) cells were untreated or treated with TNF-α (500 pg/mL), IL-1β (100 pg/mL), or HDACi 4b (25 µM) alone or in combination with poly (I:C) (1 µg/mL) or imiquimod (1 µg/mL) for 16 h prior to measuring GFP-expressing cells by flow cytometry. Fraction of cells expressing HIV is indicated by %. b Quantification of three or more combinatorial experiments is shown. Control black bars, TNF-α red bars, IL-1β blue bars, and HDACi 4b purple bars. Pam3CSK4 at 1 µg/mL and LPS at 5 µg/mL. Error bars indicate standard deviation of three or more experiments