Fig. 3.

HIV reactivation by TLR agonists in latently-infected microglial cells. Treatment of the hµglia/HIV (HC69) clonal populations with TLR ligands. HC69 cells (a) were plated 8 h before no treatment or treatment with the TLR agonists Pam3CSK4 (1 µg/mL), poly (I:C) (10 µg/mL), LPS (5 µg/mL), flagellin (5 µg/mL) or PIM₆ (5 µg/mL) for 16 h prior to measuring GFP expression by FACS analysis. THP-1/HIV (HA3) cells (b) were used as positive control