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. 2017 Feb;216(2):393–408. doi: 10.1083/jcb.201606126

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© 2017 Alleva et al.

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Figure 7. — fkb-6(tm2614) mutants show defects typical to microtubule organization defects in the germline. (A) Micronuclei in fkb-6(tm2614) mutants (grown at 25°C for 6 h). LEM-2 (nuclear envelope, green) and DAPI (red). Images are a z-stack projection halfway through the nuclei. n gonads: wild type = 16 and fkb-6(tm2614) mutants = 16. (B) Metaphase nuclei are found in higher proportion in fkb-6(tm2614) mutants. PH-3 to mark metaphase nuclei (green) and DAPI (red). Images are a z-stack projection through the nuclei at the PMT. Error bars are SD. n gonads: wild type = 8 and fkb-6(tm2614) mutants = 8. (C) Microtubule defects found in embryos, as indicated in the images, distributed to categories in wild-type (n = 68) and fkb-6(tm2614) mutants (n = 31). (D) Nuclear positioning defects in fkb-6(tm2614) mutants, SYP-1 (red) and DAPI (blue). Images are a z-stack projection of the top and bottom layer. Bars, 2 μm. (E) Quantification of microtubule density around the nucleus by three methods (ImageJ). Box-and-whisker plots are made with minimum to maximum values. n nuclei: wild type = 12 and fkb-6 = 12 for all. *, P < 0.05. (F) Model.