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. 2017 Feb;216(2):379–391. doi: 10.1083/jcb.201608043

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© 2017 D'Archivio and Wickstead

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 6. — KKIP1 is an outer kinetochore protein. (A) Localization of KKT and KKIP components in cells at 0 or 8 h after induction of KKIP1 RNAi. (B) YFP-KKIP1 shows incomplete colocalization with predicted inner kinetochore components. (C) Subpixel positions of peak intensity for mStrawberry-tagged KKTs and KKIPs in anaphase cells. Positions of foci in individual cells (red dots) are shown relative to YFP-KKIP1 peak (green crosses) with spindle axis aligned to x. N gives number of independent spindles contributing to positions shown. (D) Distribution of relative positions along spindle axis. Negative values represent positions toward the mid-spindle. (E) Mean relative positions. Error bars show SEM (***, P < 0.001; Student's t test). Bars, 4 µm.