Figure 4. Western blotand real-time PCR results show simultaneous activation of redox signaling molecules and UPR signaling.

(A) Western blot result showsupregulation of UPR associated proteins CHOP and HSP70 and Redox associated proteins Peroxiredoxin 3 and Catalase. (B) mRNA levels ofthe UPRgenesin breast cancer cells MDA-MB-231 cells were analyzed by quantitative realtime PCR (expression was normalised to L19 RNA expression). Experiments were performed in triplicate. Data are expressed with a standard deviation from the mean (n = 3). Our results shows fold induction of UPR associated genes CHOP and HSP70 and redox associated genes PRDX3 and catalase.^**P < 0.01, compared with the Light control.