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. 2017 Feb 7;7:42126. doi: 10.1038/srep42126

Figure 7. Apoptotic induction of diiodo-squaraine in PDT.

Figure 7

(A) Annexin Flow cytometric analysis of MDA-MB-231 cells after PDT treatment with light control and dark control and in presence of diiodo-squaraine at concentration 10 μM and 20 μM. Data are expressed as a mean value ± standard deviation of three independent experiments. The lower left quadrant (Q3) of the panel shows the viable cells, negative for both Annexin V−FITC and PI. The lower right quadrants (Q4) represent the early apoptotic cells Annexin V−FITC and Propidium iodide (PI). The upper right quadrants (Q2) represent the late apoptotic cells positive for Annexin V−FITC and Propidium iodide (PI). Our results shows that cells undergoing early and late apoptosis on diiodo-squaraine PDT was exclusively concentration dependent (B) Western blot analysis shows activation of caspase 3, 7, 9 followed by PARP cleavage (C) DNA ladder formation using 10 and 20 μM diiodo-squaraine PDT confirms that DNA fragmentation was concentration dependent.