Fig. 3.

Trabectedin displaces EWS-WT1 chimera from its target gene promoters. a ChIP assays on JN-DSRCT-1, treated and not with trabectedin 5 nM for 1 h, 0.5 nM and 0.75 nM for 24 h. The binding of EWS-WT1 on IGF2, PDGFA and EGFR was analyzed by RT-Q-PCR. Data are shown according to the Fold enrichment method using PRISM GraphPad software. * p value < 0.005. b ChIP assays on JN-DSRCT-1, treated and not with trabectedin 5 nM for 1 h, and recovered after 6 and 24 h. The binding of EWS-WT1 on EGFR and ENT-4 was analyzed by RT-Q-PCR. Data are shown according to the Fold enrichment method using PRISM GraphPad software. * p value < 0.005