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. 2017 Feb;214(2):459–473. doi: 10.1084/jem.20160592

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© 2017 Lang et al.

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Figure 8. — TRIM65-mediated MDA5 oligomerization depends on MDA5 ubiquitination at lysine 743. (A) Confocal microscopy analysis of HeLa cells cotransfected with plasmids for GFP-TRIM65, mCherry-MDA5, or MDA5 mutant (K743R). The third and sixth columns show higher-magnification images of white boxes in the second and fifth columns, respectively. Bars: (white) 20 µm; (red) 2 µm. (B) Immunoblot analysis of MDA5 in HEK-293T cells transfected with constructs encoding various combinations of GFP-TRIM65, MDA5, or MDA5 mutant (K743R) by SDD-AGE or SDS-PAGE assay. Data are representative of three independent experiments.