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. 2017 Feb;214(2):475–489. doi: 10.1084/jem.20161312

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© 2017 Chen et al.

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Figure 2. — SFR deficiency blocks NKT cell development and PLZF induction at an early stage. (A) Flow cytometric analysis of SFR expression on developing CD3⁺CD1d-Tet⁺ NKT cells in the WT thymus, including CD24^hi (stage 0), CD24^lowCD44^lowNK1.1⁻ (stage 1), CD24^lowCD44^hiNK1.1⁻ (stage 2), and CD24^lowCD44^hiNK1.1⁺ (stage 3) cells. The corresponding SFR-deficient cells were used as a negative control. The data are representative of three experiments. (B) Representative flow cytometry plots (left) and percentages (right) of cells in the four stages of development among gated CD3⁺CD1d-Tet⁺ NKT cells in the thymus of the indicated mice, S0: stage 0; S1+2+3: stage 1+2+3. Data represent the mean ± SEM of 4–10 mice per group. *, P < 0.05; ***, P < 0.001. (C) Flow cytometric analysis (left) and mean fluorescence intensity (MFI; right) of PLZF expression on the CD3⁺CD1d-Tet⁺ NKT cells at the four development stages in the thymus of the indicated mice. Data represent the mean ± SEM of four to seven mice per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (D) Flow cytometric analysis of Egr2 expression on the CD3⁺CD1d-Tet⁺ NKT cells at the four development stages in the thymus of the indicated mice. The data are representative of three experiments.