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. 2017 Feb;214(2):475–489. doi: 10.1084/jem.20161312

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© 2017 Chen et al.

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Figure 7. — SFR deficiency does not affect humoral immunity. (A) Flow cytometry analysis of SFR expression in resting splenic B cells (CD19⁺) and CD4 T cells (CD4⁺CD19⁻) from nonimmunized WT mice as well as GC B cells (CD19⁺GL7^hiFas^hi) and GC T_FH cells (CD4⁺CD19⁻CXCR5^hiPD1^hi) from SRBC-immunized WT mice. Respective SFR-deficient cells were used as negative controls. The data are representative of three experiments. (B and C) Representative flow cytometry plots (left) and percentages (right) of splenic GC B cells (CD19⁺Fas^hiGL7^hi), plasma cells (CD19⁺IgD^loCD138^hi), and GC T_FH cells (CD4⁺CD19⁻PD1^hiCXCR5^hi) from WT and SFR KO mice 7 d after SRBC immunization (B) or 9 d after OVA immunization (C). Data represent the mean ± SEM of 9–25 mice per group. **, P < 0.01. (D) Percentage of T–B conjugation (number of CD4⁺CD19⁺ cells out of total number of CD4⁺ cells). OT-II CD4⁺ T cells from WT and SFR-deficient mice and B cells were pulsed with OVA_323–339 peptide at the indicated concentrations. The data are representative of three experiments and show the mean ± SEM of three mice per group.