Fig. 3.

TAGs display a distinct gene expression profile. a Boxplot showing the normalised fluorescence intensities of the labelled samples that were analysed (upper left panel). Global hierarchical clustering of the samples (lower left panel) shows that samples from normal glial cells and TAGs from non-angiogenic and mature GBM phenotypes group separately. Global hierarchical clustering analysis over both genes and samples (right panels) show TAG gene clusters, both over- and underexpressed compared to normal glial cells. b Mean normalised expression profiles of “stemness” genes are upregulated in TAGs. c The numbers of significantly overexpressed genes in various groups are shown in their respective compartments in the non-overlapping areas (SAM analysis, q < 0.05). Genes that are not significantly differentially expressed are shown in the overlapping areas