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Effects of miR-203 and miR-320b on nodule formation and ALP activity. Human osteoblast cells were cultured and were transfected with miR-203, miR-320b miRs or Anti-miRs and with negative control (NC), and cells were stimulated with BMP-2 for 12 days. The deposition of calcium was detected by: (A) Alizarin Red staining; and (B) an ALP staining assay. Experiments were performed in triplicate and representative micrographs are shown with scale bar = 200 µm.
