Figure 6.

Characterization and functional analyses of the Dlx5 3′ UTR. (A) Schematic representation of dual luciferase reporter plasmid psiCHECK-2 vector containing the 3′ UTR of Dlx5 inserted downstream of Renilla luciferase gene. Four types of dual luciferase reporter plasmids were constructed, one wild type and three types of mutations were constructed within the seed region of the miR-203 and miR-320b binding site. (B) Effect of miR-203 and miR-320b overexpression on the plasmid containing the 3′ UTR of Dlx5 with the mutations was analyzed. Firefly and luciferase activity of each construct were measured in cell lysates. The luciferase activity was normalized to the miRNA negative control (NC). All values are represented as mean ± S.D. of three independent experiments. Statistical significance is indicated as and ** p < 0.001.