Figure 2.

Duration of exposure effects on pRNAi response using brahma (brm) and hunchback (hb) dsRNA in D. v. virgifera. Females were fed with diet treated with dsRNA; the number following T indicates the number of times that females received dsRNA (2 μg per diet pellet), diet provided every other day for twelve days. Diet treated with water and GFP dsRNA provided six times were used as controls. (a) Eggs collected from dsRNA-fed females after last feeding exposure; (b) Eggs hatched based on numbers oviposited; (c,d) Relative transcript level for brahma (brm) and hunchback (hb) in D. v. virgifera females. T indicates the number of times that females received dsRNA (2 μg per diet pellet), diet provided every other day for twelve days. Comparisons performed with Dunnett′s test (control group = water), * significance at p < 0.05. ** significance at p < 0.001.