Figure 3.

Timing of exposure effects on pRNAi response using brahma (brm) and hunchback (hb) dsRNA in D. v. virgifera. Females were fed diet with 2 μg dsRNA six times before mating, six times immediately after mating, and six times six days after mating. Diet treated with water and GFP dsRNA were used as controls. (a) Eggs collected from dsRNA-fed females after last feeding exposure; (b) Eggs hatched based on the numbers oviposited. Comparisons performed with Dunnett′s test (control group = water), * significance at p < 0.05. ** significance at p < 0.001.