Figure 2.

Assay for siRNA inhibition of the VdAAC gene. V. dahliae protoplasts were transformed with 10 μM siRNA-1, siRNA-2, siRNA-3, siRNA-4 or control, respectively. After regenerating for 18 h in TB3 broth, the protoplasts were cultured in the center of a PDA plate. (A) Colony morphology on PDA after 2 weeks; (B) Relative expression levels of VdAAC in different RNAi-treated groups. RNA was extracted from mycelia 72 h after transformation. First strand cDNA was synthesized, and qRT-PCR was carried out; (C) Number of conidia produced by the control and siRNA groups after 2 weeks. Error bars represent standard deviation (SD) calculated from means for three independent replicates. Significant differences (p < 0.05) among means for the different incubation times in Duncan’s multiple range test are indicated with different letters.