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. 2017 Jan 14;8(1):34. doi: 10.3390/genes8010034

Figure 1.

Figure 1

Site-directed RNA editing with R/G-guideRNAs. (A) Scheme of the three human ADARs used in this study; (B) Design principle: the R/G-guideRNAs have been developed as trans-acting guideRNA from the natural cistronic R/G-motif of the GluR2 transcript. The binding sites of the dsRBDs (dsRNA-binding domains) of ADAR2 are indicated; (C) Sanger sequencing of the editing experiments when applying the R/G-guideRNA with ADAR1p110 or p150 compared to ADAR2 in the repair the W58x codon in eGFP. Shown is the sequence around the editing site (arrow) and around a typical off-target site, A381 (*). For full Sanger sequences see Figures S2 and S3.