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Effect of ribose and deoxyribose backbone on the removal of dU by SacUDGs. SacUDGIV (a) and SacUDGV (b) were incubated with 0.1 μM single-stranded (DNA-dU-DNA, RNA-dU-RNA) and double-stranded substrates (DNA-dU-DNA/DNA, DNA-dU-DNA/RNA, RNA-dU-RNA/DNA, and RNA-dU-RNA/DNA) at 50 °C for 10 min. After incubation, 10 mM NaOH (final concentration) was added to reaction and heated for 10 min at 95 °C to cleave the AP sites. The amount of each UDG is 2 ng (a), and 10 ng (b), respectively.
