Table 3.

Hepatic relative PC^# and PCK1 mRNA expression during the periparturient period in cows supplemented with or without rumen-protected MET and CHOL.

	MET		CHOL			Day				p-Value 2
Genes	Without	With	Without	With	SEM 1	−10	7	20	30	MET 3	CHOL 4	Time	M × T 5	C × T 6
PC	2.12	2.02	2.09	2.05	0.07	1.49 c	2.88 a	2.23 b	1.93 d	0.33	0.70	<0.01	0.28	0.34
PCK1	2.27	2.43	2.39	2.31	0.10	1.57 c	2.62 ab	2.74 a	2.69 ab	0.27	0.55	<0.01	0.48	0.46

* Significant (p < 0.05) parity effect observed. Labeled means in a row without a common superscript letter differ (p < 0.05). ^# PC = pyruvate carboxylase; PCK1 = Phosphoenolpyruvate carboxykinase 1; ¹ Greatest SEM; ² MET × CHOL interaction was included in the supplemental file; ³ Overall effect of MET supplementation; ⁴ Overall effect of CHOL supplementation; ⁵ Interaction of MET × time; ⁶ Interaction of CHOL × time.