Figure 1.
Outline of the tracer infusion and blood sampling protocols. After 15 min of blood sampling at baseline, pigs were fed at hourly intervals with intravenous infusion of NaH13CO3 for 2 h followed by a 6-h infusion of [1-13C]leucine. Blood samples were obtained every 1 h throughout the 8-h tracer infusion. One hour before the first feeding, an infusion of p-amino hippurate (PAH) was made and continued throughout the experimental period.