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. 2017 Jan 15;9(1):31. doi: 10.3390/nu9010031

Figure 5.

Figure 5

Roles of ERK1/2 and p38 MAPK in GHP-induced Nrf2 activation and HO-1 expression in HepG2 cells. (A) Cells were pretreated with PD98059 (20 μM) or SB203580 (20 μM) for 2 h prior to incubation with GHP (2.0 mg/mL) for 12 h. Cells were harvested and HO-1 expression was determined by Western blot analysis; (B) Cells were pretreated with either PD98059 or SB203580, each at a concentration of 20 μM, for 2 h prior to incubation with GHP (2.0 mg/mL) for additional 12 h. Then nuclear and cytosol levels of Nrf2 were measured by Western blot analysis. The results are presented as the means ± SD of three independent experiments. Means with different letters are significantly different from each other at p < 0.05.