Table 3.
The fatty acid profiles of different lipid fractions in spleen tissues (mg/g dry spleen biomass).
| Neutral Lipids | Control n = 5 | HFD-ED n = 8 | HFD-CO n = 8 |
|---|---|---|---|
| C12:0 | 0.00 ± 0.00 | 0.01 ± 0.00 | 0.06 ± 0.02 |
| C14:0 | 0.01 ± 0.00 | 0.01 ± 0.00 a | 0.14 ± 0.06 b |
| C16:0 | 1.03 ± 0.20 | 1.46 ± 0.22 | 3.34 ± 1.05 |
| C18:0 | 0.21 ± 0.02 | 0.19 ± 0.04 | 0.13 ± 0.03 |
| C18:1 n-9 | 1.78 ± 0.46 | 1.05 ± 0.17 | 1.43 ± 0.33 |
| C18:2 n-6 | 0.03 ± 0.02 a | 0.2 ± 0.08 a | 0.86 ± 0.25 b |
| C20:3 n-6 | 0.11 ± 0.01 a | 0.03 ± 0.01 b | 0.05 ± 0.02 |
| C20:4 n-6 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.24 ± 0.14 |
| C22:6 n-3 | 0.00 ± 0.00 a | 0.16 ± 0.04 b | 0.00 ± 0.00 a |
| Total | 3.16 ± 0.58 | 3.12 ± 0.43 | 6.25 ± 1.37 |
| Free fatty acids | |||
| C12:0 | 0.01 ± 0.00 a | 0.02 ± 0.00 | 0.04 ± 0.01 b |
| C14:0 | 0.01 ± 0.00 | 0.02 ± 0.00 | 0.05 ± 0.01 |
| C16:0 | 0.20 ± 0.02 | 0.20 ± 0.01 | 0.35 ± 0.07 |
| C18:1 n-9 | 0.05 ± 0.00 | 0.05 ± 0.00 a | 0.06 ± 0.00 b |
| C18:3 n-3 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.03 ± 0.00 |
| C20:3 n-6 | 0.06 ± 0.01 | 0.02 ± 0.00 a | 0.07 ± 0.01 b |
| C22:6 n-3 | 0.00 ± 0.00 a | 0.05 ± 0.00 b | 0.00 ± 0.00 a |
| Total | 0.35 ± 0.03 | 0.38 ± 0.02 | 0.60 ± 0.11 |
| Phospholipids | |||
| C12:0 | 0.00 ± 0.00 a | 0.01 ± 0.00 | 0.01 ± 0.00 b |
| C14:0 | 0.07 ± 0.00 a | 0.15 ± 0.01 b | 0.13 ± 0.00 c |
| C16:0 | 1.69 ± 0.05 | 1.70 ± 0.03 | 1.69 ± 0.05 |
| C18:0 | 0.61 ± 0.02 | 0.59 ± 0.01 | 0.67 ± 0.04 |
| C18:1 n-9 | 0.32 ± 0.01 a | 0.28 ± 0.01 b | 0.31 ± 0.01 a |
| C18:2 n-6 | 0.12 ± 0.00 a | 0.22 ± 0.02 b | 0.14 ± 0.01 a |
| C20:0 | 0.00 ± 0.00 | 0.01 ± 0.00 | 0.00 ± 0.00 |
| C20:1 n-7 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.02 ± 0.00 |
| C20:2 n-6 | 0.02 ± 0.00 a | 0.05 ± 0.01 b | 0.03 ± 0.00 a |
| C20:3 n-6 | 0.17 ± 0.01 a | 0.18 ± 0.01 a | 0.25 ± 0.02 b |
| C20:4 n-6 | 4.41 ± 0.10 a | 1.92 ± 0.05 b | 4.33 ± 0.08 a |
| C20:5 n-3 | 0.00 ± 0.00 a | 0.41 ± 0.01 b | 0.00 ± 0.00 a |
| C22:0 | 0.02 ± 0.01 | 0.02 ± 0.00 | 0.01 ± 0.00 |
| C22:5 n-3 | 0.00 ± 0.00 a | 0.34 ± 0.01 b | 0.00 ± 0.00 a |
| C22:6 n-3 | 0.20 ± 0.02 a | 0.82 ± 0.02 b | 0.19 ± 0.01 a |
| Total | 7.67 ± 0.19 a | 6.71 ± 0.13 b | 7.78 ± 0.22 a |
The fatty acid profiles from the mice fed either control, HFD-ED or HFD-CO are shown as mean ± SEM and as a proportion of total fatty acid fraction; different letters show statistical difference tested by ANOVA followed by Tukey’s multiple comparison test. For details see methods section.