Table 1.
Experimental diets (g/100 g).
| Ingredient | GSO | CO | CNO | Ingredient | GSO | CO | CNO |
|---|---|---|---|---|---|---|---|
| GSO 1 | 10.0 | DL-methionine 2,4 | 0.2 | 0.2 | 0.2 | ||
| CO 1 | 10.0 | Cellulose 2 | 5.0 | 5.0 | 5.0 | ||
| CNO 1 | 10.0 | AIN-93G-Mineral mix 2 | 3.5 | 3.5 | 3.5 | ||
| SFA | 1.1 | 1.4 | 9.2 | AIN-93-Vitamin mix 2 | 1.0 | 1.0 | 1.0 |
| MUFA | 3.2 | 3.2 | 0.7 | Choline chloride 5 | 0.02 | 0.02 | 0.02 |
| PUFA | 5.7 | 5.4 | 0.1 | Sucrose + maltodextrins 1 | 17.6 | 17.7 | 17.7 |
| Casein 2,3 | 21.0 | 21.0 | 21.0 | Corn starch 1 | 38.7 | 38.8 | 38.8 |
1 Food-grade (several trademarks); grapeseed oil (GSO), corn oil (CO), coconut oil (CNO); 2 food-grade from Bioserv, Inc. (Frenchtown, NJ, USA); 3 ANRC (American National Research Council) grade: 95% protein, vitamin-free, 2.5 of total sulfur amino acids (TSA)/100 g protein; 4 to reach >0.98 g/100 g diet of TSA [29]; 5 99% pure (74.6% choline); saturated (SFA), mono- (MUFA) and poly-unsaturated fatty acids (PUFA). All diets were isocaloric (4 kcal/g).