Table 3.
LINE1 methylation pyrosequencing assay
| Criteria for comparison | Groups (n) | Global methylation fraction (mean ± SD) | P value | |
|---|---|---|---|---|
| ANOVA | Tukey’s post hoc test (vs controls) | |||
| Controls (65) | 0.4997 ± 0.0463 | – | – | |
| Total ART | ART (126) | 0.4789 ± 0.0459 | – | 0.0034 a |
| Mode of egg fertilization | ICSI (39) | 0.4732 ± 0.0322 | 0.0090 | < 0.05 |
| Non ICSI (87) | 0.4815 ± 0.0508 | < 0.05 | ||
| Oxygen tension | 20%(73) | 0.4781 ± 0.0526 | 0.0135 | < 0.05 |
| 5%(53) | 0.4799 ± 0.0352 | ns | ||
| Type of embryo transfer | Fresh (90) | 0.4784 ± 0.0487 | 0.0137 | < 0.05 |
| Frozen (36) | 0.4800 ± 0.0385 | ns | ||
| Embryo transfer day | Day 3 (67) | 0.4783 ± 0.0529 | 0.0137 | < 0.05 |
| Day 5 (59) | 0.4796 ± 0.0463 | < 0.05 | ||
ANOVA analysis of variance; ART assisted reproductive technology; SD standard deviation; ICSI intracytoplasmic sperm injection; ns non significant
aUnpaired two-tailed t test
Values in italics denote significance