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. 2017 Jan 4;15(1):7. doi: 10.3390/md15010007

Figure 5.

Figure 5

Effect of PD1 on the inductions and secretions of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) (A); nitric oxide (NO) (B); tumor necrosis factor α (TNF-α) (C); interleukin -1β (IL-1β) (D); and interleukin-6 (IL-6) (E). RAW264.7 macrophages were pre-treated with the indicated concentrations of PD1 or dexamethasone (DEX) for 1 h and then with LPS (1 μg/mL, 24 h), and iNOS and COX-2 levels were determined by Western blot. RAW264.7 macrophages were pre-treated with PD1 or dexamethasone (DEX) for 1 h and then with LPS (25 ng/mL, 24 h), and NO concentration in medium were determined by Griess method, and the expression levels of IL-1β, IL-6, and TNF-α in medium were determined by enzyme-linked immunosorbent assay (ELISA). In the case of TNF-α, cells were pre-treated with PD1 or dexamethasone for 1 h and then with LPS (25 ng/mL, 3 h). The results shown are representative of three independent experiments. ## p < 0.01, ### p < 0.001 vs. untreated controls. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. LPS-treated cells.