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Phosphorylated nuclear factor kappa B (NF-κB) p65 levels in RAW264.7 murine macrophages were determined by Western blot using β-actin as the internal control. Cells were pretreated with PD1 (50 μM) or dexamethasone (DEX, 50 μM) for 30 min, and stimulated with 20 ng/mL LPS for 15 min. ### p < 0.001 vs. untreated controls. * p < 0.05 and ** p < 0.01 vs. LPS-treated cells.
