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Purification of wild-type MaBGA (β-galactosidase from Marinomonas sp. BSi20414). (a) Ion exchange chromatography. Peak I was unbound proteins; Peak II and III were proteins eluted by 0.1–0.2 M of NaCl; Peak IV was protein eluted by 0.20–0.24 M of NaCl; Peak V was protein eluted by 0.24–0.6 M of NaCl; (b) SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis of purified MaBGA. Lane M: protein molecular weight marker; Lane E: purified MaBGA.
