Table 1.
Purification Steps | Total Protein (mg) | Total Activity (U) | Specific Activity (U/mg) | Recovery (%) | Purification Fold |
---|---|---|---|---|---|
Cell lysate | 162.54 | 1818.18 | 11.19 | 100 | 1 |
Ammonium sulfate precipitation | 29.9 | 927.27 | 31.01 | 51 | 2.77 |
HiTrap DEAE FF | 1.01 | 91.80 | 90.89 | 9.9 | 8.12 |