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. 2016 Aug 15;7(36):57498–57513. doi: 10.18632/oncotarget.11292

Figure 2. KB promotes LPS absorption by HCs and inhibits LPS uptake by KCs.

Figure 2

A. C57 BL/6 mice were injected with FITC-LPS (1 mg/kg) preincubated with KB (3.6 mg/kg) for 1h. Colocalization of FITC-LPS (green) with cell markers for HCs, KCs, HSCs and LSECs (red) was detected in frozen liver sections by confocal microscopy (objective×40). Nuclei were stained with DAPI (blue). B. C57 BL/6 mice were injected with NS or Clodronate liposomes for KC depletion, followed by 1 mg/kg FITC-LPS preincubated with 3.6 mg/kg KB. Hepatic uptake of LPS and its colocalization with HCs and KCs in liver sections were detected. C. & D. Primary HCs (2×105/ml), HepG2 (2×105/ml) cells, primary KCs (1×106/ml) and RAW 264.7 cells (1×106/ml) were treated with FITC-LPS (1 μg/ml) preincubated with H2O or KB (15 μM) for 1h. LPS uptake was detected by flow cytometry and expressed as relative fluorescence (Relative FL). *P < 0.05 vs FITC-LPS.