Figure 3. KB induced LPS uptake in hepatocytes independent of TLR4.

A. TLR4^−/− mice were injected with NS and FITC-LPS (1 mg/kg) preincubated with NS or KB (3.6 mg/kg) for 1 h. Distribution of FITC-LPS on frozen liver sections was detected by confocal microscopy. B. FITC-LPS fluorescence in whole liver tissues is expressed as means ± SD (n = 3). *P < 0.05 vs. FITC-LPS. C. FITC-LPS uptake in HCs isolated from TLR4^−/− mice was analyzed by flow cytometry and expressed as relative fluorescence (Relative FL). *P < 0.05 vs FITC-LPS. D. HCs from WT C57BL/6 mice were treated with LPS (1 μg/ml) preincubated with or without KB (15 μM) for 4 h. TLR4 expression was detected by real-time PCR. E. HCs (2×10⁵/ml) from WT mice were treated with FITC-KB (15 μM) for 30 min. KB (green) and TLR4 (red) colocalization was detected by immunofluorescence.