Figure 2. Peribronchial morphological changes after chronic OSA in the lung section of canines.

(A) Hematoxylin and eosin (H&E) staining, scale bar=50μm; (B) Transmission electron microscope images of representative alveolar epithelial cell aside the bronchia, the arrow indicates pinocytotic vesicles, vesicular nuclei and nuclear membrane change in the alveolar epithelial cells, scale bar=2μm; (C) Masson's trichrome staining of representative peribronchial lung section from different groups and quantitative calculation of collagen volume fraction percentage, scale bar=50μm; (D) Immunohistochemistry staining of Collagen type I and Western Blot analysis of protein lysates from the tissue of canines in different groups (E), scale bar=50μm.