Figure 4. Sustained MYC expression is required for savolitinib resistance.

A., cell viability following MYC knockdown ± savolitinib treatment. Cells were transfected with control siRNA (siSCR) or MYC-targeting siRNAs (siMYC^A, siMYC^B) and treated with vehicle or 100 nM savolitinib for 72 hours. Data shown are the mean ± S.D. of three replicates. B., viability of parental H1993 cells stably expressing a doxycycline-inducible RFP (negative control) or human MYC. Cell viability was assessed after 24 hours of doxycycline treatment followed by vehicle or savolitinib treatment for 24 hours. C., immunoblot validation of MET inhibition and MYC overexpression for a single savolitinib dose (100 nM). Vinculin serves as a loading control. D., a schematic consistent with our data summarizing the putative role of MYC deregulation in savolitinib resistance.