Figure 4.

Quantitation of the number of rRNA transcripts per gene in chromatin spreads indicates that depletion of the t-Utps leads to a reduction in pre-rRNA transcription. Each strain carries the gene encoding the indicated protein under the control of a GAL promoter, allowing for conditional expression. The parent strain is YPH499. The boxes indicate t-Utps. (A–D) Examples of chromatin spreads from the indicated strains. The Mpp10, Utp4, and Utp10 proteins were depleted by growth in glucose for 6 h. (E) Results from quantitation of the number of rRNA transcripts per gene upon depletion of the indicated proteins. The average number of transcripts per gene for 32 genes for each strain is shown ± standard deviation.