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. 2004 Oct 15;18(20):2506–2517. doi: 10.1101/gad.1226604

Figure 7.

Figure 7.

(A) The t-Utps coimmunoprecipitate pre-18S rRNAs. The indicated proteins were HA-tagged by integration of the tag at the genomic locus. Boxes indicate t-Utps. “B” indicates immunoprecipitations performed with beads alone (no antibody), “IP” indicates immunoprecipitations performed with anti-HA antibody. Coimmunoprecipitating RNAs were isolated and analyzed by Northern blot with oligonucleotides complementary to the pre-rRNAs (oligos c, b, and e; Fig. 1). Pre-rRNA species were determined by their size relative to total RNA extracted by the hot phenol method from the parental YPH499 strain (data not shown). (B) The t-Utps coimmunoprecipitate pre-18S rRNAs in the absence of the U3 snoRNA. The indicated t-Utps were HA-tagged in the YKW100 strain. Anti-HA immunoprecipitations were performed on extracts from yeast depleted (D) or not (U) of the U3 snoRNA by growth in glucose. Coimmunoprecipitating RNAs were isolated and analyzed by Northern blot with an oligonucleotide complementary to pre-rRNAs (oligo c; Fig. 1).