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. 2016 Jul 27;7(36):57783–57797. doi: 10.18632/oncotarget.10860

Figure 5. SATB2 shRNA inhibits cell proliferation and colony formation in pancreatic cancer cells and Pan CSCs.

Figure 5

(A) Expression of SATB2. PANC-1, AsPC-1 and Pan CSCs were transduced with either scrambled or SATB2 shRNA. Cell lysates were collected and the expression of SATB2 was measured by the Western blot analysis. β-actin was used as a loading control. (B) Cell proliferation of PANC-1/Scrambled, PANC-1/SATB2 shRNA, AsPC-1/Scrambled, AsPC-1/SATB2 shRNA, Pan CSCs/Scrambled and Pan CSCs/SATB2 shRNA groups was measured over 6-day period. (C) Colony formation Assay. PANC-1/Scrambled, PANC-1/SATB2 shRNA, AsPC-1/Scrambled, AsPC-1/SATB2 shRNA, Pan CSCs/Scrambled and Pan CSCs/SATB2 shRNA cells were seeded, and number of colonies formed at 21 days were counted. Data represent mean (n = 4) ± SD. * = significantly different from HPNE empty vector (P < 0.05).