Table 5. Primers and PCR programs for DcR3 PCR-RFLP genotyping.
SNP | primer | restriction enzyme | PCR products length |
---|---|---|---|
Rs3208008 | F: 5′- GTAGCTGACTCCTGAACCG-3′ R: 5′- GGTCGTCGTCTTGCTTATAG-3′ |
CspCI | 417 bp |
Rs41309931 | F: 5′-AGGGTTCAGCATGTTTGTG-3′ R: 5′-ATCTTGCTCTGGGTCTTCC-3′ |
BanI | 307 bp |
Rs2297441 | F: 5′-ACCCACCCAACAGAATAGGC-3′ R: 5′-AAGTACAGTGTCACATGGCAG-3′ |
BbvI | 410 bp |
Rs2257440 | F: 5′-AAAGGAGGTGGCATGTCG-3′ R: 5′-AGAGGACGTTGCAGTAGCG-3′ |
HinP1I | 363 bp |
Rs909341 | F: 5′- CGCTGGTTTCTGCTTGGAG-3′ R: 5′-GATTGCTGCCCACTTTCC-3′ |
BpmI | 640 bp |
Rs1291207 | F: 5′- GGCCTGATGGTAACTCTCC-3′ R: 5′- AGAGAGAAATCCCTGTGCAG-3′ |
BsrBI | 351 bp |