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. 2016 Aug 9;7(36):58065–58074. doi: 10.18632/oncotarget.11161

Figure 4. Impaired mitophagy in FA cells is dependent on mitochondrial fission.

Figure 4

(A) Immunoblots of possible autophagic markers. (B) Immunoblots of mitochondrial and cytoplasmic fractions of FA cells. (C) Fractions of FA cells transfected with wtDRP1 or dnDRP1 were enriched by FACS-sorting, treated with CCCP for indicated time intervals and transfected with LC3-RFP. Representative graph showing the average number of puncta signals in FANCA cells. *P < 0.05, **P < 0.03 The plasmids CMV_DRP1_IRES_mitoDsRed andCMV_DRP1dn_IRES_mitoDsRed expressing wild type and dominant negative DRP1 (DRP1dn = DRP1K38A). (D) Corresponding cell samples were processed for immunoblot analyses and the average signal intensities of LC3-II/I bands were plotted on a diagram.