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. 2016 Aug 9;7(36):58089–58104. doi: 10.18632/oncotarget.11166

Figure 2. NaHS reverses hypoxia--ischemic induced changed of Bax and Bcl-2 and caspase-3 activation in BMSCs in vitro.

Figure 2

(A–B) BMSCs exposed to hypoxia-ischemic were incubated in the absence or presence of NaHS (1 μM) for 72 h. The protein and mRNA levels of Bax and Bcl-2 were then analyzed by Western blot (A) and by semiquantitative RT-PCR (B). Levels of β-actin were used to evaluate protein loading. Results were expressed as Bax/Bcl-2 ratio. (C) BMSCs exposed to hypoxia-ischemic were incubated in the absence or presence of NaHS (1 μM) for 72 h. The cells extracts were subjected to Western blot analysis using an antibody against cleaved caspase-3, Levels of caspase-3 were used to evaluate protein loading. Quantification of the protein levels of cleaved caspase-3 and caspase-3 as determined by Image-Pro Plus 6.0. Values represent the mean ± SD of n = 4. **p < 0.01 Hy VS Con; ##p < 0.01 Hy+ NaHS VS Hy.