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. 2016 Aug 5;7(36):58315–58330. doi: 10.18632/oncotarget.11089

Figure 4. ω-3 PUFAs inhibit proliferation and induce apoptosis of CRC cells via YAP.

Figure 4

A. LOVO cells were infected with empty vector or pQCXIH Myc-YAP (5SA) retroviral for 72 h, after infection, cells were treated with 75 μM DHA or EPA for additional 48 h, cell apoptosis was determined by FACS analysis. B and C. CRC cells were infected with empty vector or pQCXIH Myc-YAP (5SA) retroviral for 72 h, after infection, cells were treated with 75 μM DHA or EPA for 24h, 48h, 72h, or 96h respectively, cell viability was determined by MTT assay. D and E. LOVO cells were transfected with YAP siRNA for 48 h. After transfection, cells were treated with 75μM DHA or EPA for additional 24h. Total RNA was extracted and used for qRT-PCR analysis of the representative panel of pro-proliferative genes and anti-apoptosis genes. F and G. LOVO cells were infected with empty vector or pQCXIH Myc-YAP (5SA) retroviral for 72 h. After infection, cells were treated with 75 μM DHA or EPA for additional 24 h. Total RNA was extracted and used for qRT-PCR analysis of the representative panel of pro-proliferative genes and anti-apoptosis genes. The data are expressed as the mean ± SEM for triplicate experiments. *P<0.05.