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. 2016 Aug 6;7(36):58331–58350. doi: 10.18632/oncotarget.11110

Figure 9. ASK in vivo decreases lung metastasis of B16F10 melanoma induced by tail vein injection of cells.

Figure 9

A. Scheme of the assay. Eight C57BL/6 mice were inoculated through the tail vein with 100,000 B16F10 cells. Mice were randomized into two groups of 4 mice each, which received 6 ip injections of 250 μl saline containing 100 μg of ASO-C or ASO-1560S on days 7, 9, 11, 13, 15 and 18 post-cell injection. On day 21 mice were sacrificed and lungs were collected and fixed in formalin. B. Lungs from mice treated with ASO-C exhibited multiple metastasis nodules of different sizes. In contrast, the lungs from ASO-1560S-treated mice treated displayed fewer and smaller nodules. C. Total weight of lungs from ASO-1560S-treated mice was comparable to control mice that had not been inoculated with B16F10 cells (non-inoculated controls; NIC), while the lungs of ASO-C-treated mice displayed an average weight increase of around 2.3 times.