Figure 1. GHK-Cu decreased ROS production and increased SOD activity in LPS-induced RAW 264.7 cells activation. Four hours after LPS treatment with or without GHK-Cu pretreatment for 18 h, the cells were harvested and assayed.

A. The x-axis represent the intensity of intracellular DCFDA fluorescence, and the y-axis indicate the mean number of cells. B. Total intracellular SOD activity levels were detected using a colorimetric method. The results are representative of triplicate data. The values are presented as the means ± SD (n = 3). *p < 0.05, statistically significant difference. CTL, control.