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. 2016 Aug 10;7(36):58418–58434. doi: 10.18632/oncotarget.11170

Figure 1. Construction, expression and cytotoxicity of the albumin-integrated defensins.

Figure 1

A. Schematic diagram of SnaB I/Not I gene fragments encoding for the albumin-integrated defensin DF-HSA (upper row) and HSA-DF (lower row), respectively. B. Purity analysis of the proteins DF-HSA and HSA-DF by 10% SDS-PAGE under denaturing conditions. Lane 1, molecular weight marker; Lane 2, the purified HSA-DF; Lane 3, the purified DF-HSA. C. Western blot detection of the proteins DF-HSA and HSA-DF using mouse anti-His tag monoclonal antibody (1/2000 dilution) and HRP-conjugated goat anti-mouse IgG (1/5000 dilution). Lane 1, the purified HSA-DF; Lane 2, the purified DF-HSA. D. Cytotoxicity of DF-HSA and HSA-DF respectively to MIA PaCa-2 and BxPC-3 pancreatic carcinoma cells as determined by MTT assay.