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. 2016 Aug 10;7(36):58418–58434. doi: 10.18632/oncotarget.11170

Figure 3. Cytotoxicity of the albumin-integrated defensin DF-HSA to various cancer cells.

Figure 3

A. Growth inhibition analyses of DF-HSA, free HBD2, and HSA to MIA PaCa-2, BxPC-3, A549, H460 and L02 cells, determined by the MTT assay. DF-HSA inhibited cancer cells growth in a concentration-dependent manner. B. The effect of DF-HSA and HSA on colony formation capability of MIA PaCa-2 and BxPC-3 cells was determined using clonogenic assay. C. Flow cytometry analysis of apoptosis in MIA PaCa-2 and BxPC-3 cells treated with DF-HSA, HBD2, and HSA, respectively. D. Western blot showed the levels of apoptosis associated proteins including p53, caspase-3 and cleaved caspase-3 in MIA PaCa-2 and BxPC-3 cells.